Microscopy is an essential tool for plant biologists, but new users may face many challenges when working with plant samples. A panel of experts from the Plant Cell Atlas, led by Imaging Committee co-chairs Dr. Kirk Czymmek at the Donald Danforth Plant Science Centre and Prof. Heather E. McFarlane at the University of Toronto, have put together a manual for best practices in plant fluorescence imaging and reporting. This paper offers guidance to new and intermediate microscope users with the goal of making published microscopy data better aligned with FAIR (findability, accessibility, interoperability, and reusability) principles.
Czymmek, K.J.#, Benitez-Alfonso, Y., Burch-Smith, T., Di Costanzo, L.F., Drakakaki, G., Facette, M., Kierzkowski, D., Klebanovych, A., Radin, I., Roychoudhry, S. and McFarlane, H.E.#, 2025. Best practices in plant fluorescence imaging and reporting: A primer. The Plant Cell, 37: koaf143.