Live cell imaging is an invaluable tool for understanding how cells grow, develop, and respond to stimuli. By tracking the movement of fluorescently-tagged cellulose synthase enzymes using live cell imaging, the McFarLab is able to track cell wall synthesis enzyme activity in real time in living cells.
Jana & Jenny’s paper describes a protocol for preparing samples, imaging fluorescently-tagged cellulose synthase (CESA) enzymes in live plant cells, image processing, and data analysis and has now been published in the new Cell Press journal, STAR Protocols.